Review



anti cd40  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Miltenyi Biotec anti cd40
    Anti Cd40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pm42398735-96-66-69
    Average 96 stars, based on 211 article reviews
    anti cd40 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Activation Assay:

    Article Title: Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.
    Article Snippet: T cell cytokine panel Reagent Source Catalog number Clone Dilution (PBMC, ul/180ul) Dilution (Adenoid and Tonsil, ul/180ul) 1 Anti-CD45RA-BUV395 BD 740315 5H9 0.6 ul 0.6 ul 2 Anti-CD56-BUV737 BD 612766 NCAM16.2 3.5 ul 3.5 ul 3 Anti-CD8-BUV805 BD 612889 SK1 1 ul 1.2 ul 4 Anti-CXCR5-BV750 BD 747111 RF8B2 1 ul 1 ul 5 Anti-CD25-BB515 BD 564467 2A3 10 ul 10 ul 6 Anti-CD95-BB700 BD 566542 DX2 0.6 ul 0.6 ul 7 Anti-CD103-BUV661 BD 749993 Ber-ACT8 5 ul 2.5 ul 8 Anti-CD69-BUV563 BD 748764 FN50 1 ul 1 ul 9 Anti-CXCR3-PECy5 BD 551128 1C6/CXCR3 5 ul 5 ul 10 Anti-Granzyme B-PE BD 561142 GB11 1.8 ul 3.6 ul 11 Anti-IL4-PerCP Cy5.5 BD 561234 8D4-8 7.2 ul 3.6 ul 12 Anti-IL21-Alexa Fluor 647 BD 560493 3A3-N2.1 36 ul 36 ul 13 SAv-BUV615 BD 613013 0.9 ul 0.9 ul 14 Anti-CCR7-BV421 BioLgend 353208 G043H7 5 ul 1 ul 15 Anti-CD3-BV510 BioLgend 344828 SK7 2.5 ul 2.5 ul 16 Anti-CCR6-BV711 BioLgend 353436 G034E3 1 ul 1 ul 17 Anti-PD1-BV785 BioLgend 329929 EH12.2H7 5 ul 1.2 ul 18 Anti-CD57-FITC BioLgend 359604 HNK-1 1.2 ul 1.2 ul 19 Anti-CD14-Spark Blue 550 BioLgend 367148 63D3 1 ul 2.5 ul 20 Anti-HLA-DR-APC/Fire 750 BioLgend 307658 L243 2 ul 2 ul 21 Anti-CD38-APC/Fire810 BioLgend 303550 HIT2 1 ul 1 ul 5 n atu re p o rtfo lio | rep o rtin g su m m ary M a rc h 2 0 2 1 22 Anti-CD19-Spark NIR 685 BioLgend 302270 HIB19 1.2 ul 1.2 ul 23 Anti-IL17A-BV605 BioLgend 512326 BL168 3.6 ul 3.6 ul 24 Anti-IL2-BV650 BioLgend 500334 MQ1-17H12 5.4 ul 10.8 ul 25 Anti-IFNγ-Pacific Blue BioLgend 502522 4S.B3 3.6 ul 3.6 ul 26 Anti-IL10-PE/Dazzle 594 BioLgend 506812 JES3-19F1 3.6 ul 3.6 ul 27 Anti-Perforin-APC BioLgend 353312 B-D48 9 ul 9 ul 28 Anti-TNFa-PE-Cy7 BioLgend 502930 MAb11 0.36 ul 0.36 ul 29 Anti-CD107a (LAMP-1) -Biotin BioLgend 328604 H4A3 1ul with 200ul culture medium 2 ul in 200ul culture medium 30 Anti-CD4-CD568 Cytek R7-20041 SK3 0.6 ul 2.5 ul 31 Anti-CD45-PerCP ThermoFisher MHCD4531 HI30 0.5 ul 1.2 ul 32 Anti-CD27-Super Bright 436 ThermoFisher 62-0279-42 O323 5 ul 5 ul 33 Live dead dye-Blue ThermoFisher L23105 0.225 ul 0.225 ul 5. .. Activation induced marker panel Number Reagent Source Catalog number Clone Dilution (PBMC, ul/180ul) Dilution (Adenoid and Tonsil, ul/180ul) 1 Anti-CXCR3-PE/Cy5 BD 551128 1C6/CXCR3 5 ul 5 ul 2 Anti-CXCR5-BV750 BD 747111 RF8B2 1 ul 1 ul 3 Anti-CD45RA-BUV395 BD 740315 5H9 0.6 ul 0.6 ul 4 Anti-ICOS-BUV563 BD 741421 DX29 0.6 ul 1.2 ul 5 Anti-CD56-BUV737 BD 612766 NCAM16.2 3.5 ul 3.5 ul 6 Anti-CD8-BUV805 BD 612889 SK1 1 ul 1.2 ul 7 Anti-CD40L-PE BD 557299 TRAP1 10 ul 15 ul 8 Anti-CD25-PE-CF594 BD 562403 M-A251 2 ul 2 ul 9 Anti-CCR7-BV421 BioLegend 353208 G043H7 1 ul 1 ul 10 Anti-CCR6-BV711 BioLegend 353436 G034E3 1 ul 1 ul 11 Anti-CD4-BV605 BioLegend 317438 OKT4 1 ul 1 ul 12 Anti-CD69-BV650 BioLegend 310934 FN50 2 ul 2.5 ul 13 Anti-PD1-BV785 BioLegend 329930 EH12.2H7 5 ul 1.2 ul 14 Anti-CD57-FITC BioLegend 359604 HNK-1 2 ul 1 ul 15 Anti-CD14-Spark Blue 550 BioLegend 367148 63D3 1 ul 2.5 ul 16 Anti-CD45-PerCP BioLegend 368506 2D1 0.5 ul 1.2 ul 17 Anti-4-1BB-PeCy7 BioLegend 309818 4B4-1 2.5 ul 2.5 ul 18 Anti-CD19-Spark NIR 685 BioLegend 302270 HIB19 1.2 ul 2.5 ul 19 Anti-CD38-APC/Fire 810 BioLegend 303550 HIT2 1 ul 1 ul 20 Anti-HLA-DR-APC/Fire 750 BioLegend 307658 L243 2 ul 2.5 ul 21 Anti-OX40-APC BioLegend 350008 Ber-ACT35 5 ul 5 ul 22 Live dead dye- Blue ThermoFisher L23105 0.9 ul 0.9 ul 23 CD40 Antibody, anti-human Miltenyi 130-094-133 130-094-133 0.5ug/ml 0.5ug/ml 6. .. S1+ /S1- B cell and CD95+ CD4/CD8 T cell sorting panel Number Reagent Source Catalog number Clone Dilution (PBMC, 30mill in 200ul) Dilution (Adenoid & Tonsil, 30mill in 200ul) 1 Anti-CD19-PeCy7 BIoLegend 302216 HIB19 1: 20 1: 20 2 Anti-CD56-BV510 BIoLegend 362533 5.1H11 1: 10 1: 10 3 Anti-CD14-BV510 BIoLegend 367124 63D3 1: 10 1: 10 4 Anti-CD3-Alexa Fluor 488 BIoLegend 317310 OKT3 7: 100 1: 20 5 Anti-CD8-PE BIoLegend 303804 QA18A37 1: 20 1: 20 6 Anti-CD95-PE/Dazzle 594 BIoLegend 305634 DX2 1: 40 1: 40 7 CoV2-S1-Biotin BIoLegend 793806 1: 5 of conjugated antibody 1: 5 of conjugated antibody 8 BIoLegend 405225 9 Anti-CD45-PerCP-Vio 700 Miltenyi 130-110-636 REA747 1: 20 1: 20 10 Anti-CD4-APC-eFLuor 780 ThermoFisher 47-0048-42 OKT4 1: 10 1: 10 11 Live dead dye-Aqua ThermoFisher L34957 7: 200 7: 200 12 SAv-APC ThermoFisher S32362 1: 5 of conjugated antibody 1: 5 of conjugated antibody 7.

    Marker:

    Article Title: Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.
    Article Snippet: T cell cytokine panel Reagent Source Catalog number Clone Dilution (PBMC, ul/180ul) Dilution (Adenoid and Tonsil, ul/180ul) 1 Anti-CD45RA-BUV395 BD 740315 5H9 0.6 ul 0.6 ul 2 Anti-CD56-BUV737 BD 612766 NCAM16.2 3.5 ul 3.5 ul 3 Anti-CD8-BUV805 BD 612889 SK1 1 ul 1.2 ul 4 Anti-CXCR5-BV750 BD 747111 RF8B2 1 ul 1 ul 5 Anti-CD25-BB515 BD 564467 2A3 10 ul 10 ul 6 Anti-CD95-BB700 BD 566542 DX2 0.6 ul 0.6 ul 7 Anti-CD103-BUV661 BD 749993 Ber-ACT8 5 ul 2.5 ul 8 Anti-CD69-BUV563 BD 748764 FN50 1 ul 1 ul 9 Anti-CXCR3-PECy5 BD 551128 1C6/CXCR3 5 ul 5 ul 10 Anti-Granzyme B-PE BD 561142 GB11 1.8 ul 3.6 ul 11 Anti-IL4-PerCP Cy5.5 BD 561234 8D4-8 7.2 ul 3.6 ul 12 Anti-IL21-Alexa Fluor 647 BD 560493 3A3-N2.1 36 ul 36 ul 13 SAv-BUV615 BD 613013 0.9 ul 0.9 ul 14 Anti-CCR7-BV421 BioLgend 353208 G043H7 5 ul 1 ul 15 Anti-CD3-BV510 BioLgend 344828 SK7 2.5 ul 2.5 ul 16 Anti-CCR6-BV711 BioLgend 353436 G034E3 1 ul 1 ul 17 Anti-PD1-BV785 BioLgend 329929 EH12.2H7 5 ul 1.2 ul 18 Anti-CD57-FITC BioLgend 359604 HNK-1 1.2 ul 1.2 ul 19 Anti-CD14-Spark Blue 550 BioLgend 367148 63D3 1 ul 2.5 ul 20 Anti-HLA-DR-APC/Fire 750 BioLgend 307658 L243 2 ul 2 ul 21 Anti-CD38-APC/Fire810 BioLgend 303550 HIT2 1 ul 1 ul 5 n atu re p o rtfo lio | rep o rtin g su m m ary M a rc h 2 0 2 1 22 Anti-CD19-Spark NIR 685 BioLgend 302270 HIB19 1.2 ul 1.2 ul 23 Anti-IL17A-BV605 BioLgend 512326 BL168 3.6 ul 3.6 ul 24 Anti-IL2-BV650 BioLgend 500334 MQ1-17H12 5.4 ul 10.8 ul 25 Anti-IFNγ-Pacific Blue BioLgend 502522 4S.B3 3.6 ul 3.6 ul 26 Anti-IL10-PE/Dazzle 594 BioLgend 506812 JES3-19F1 3.6 ul 3.6 ul 27 Anti-Perforin-APC BioLgend 353312 B-D48 9 ul 9 ul 28 Anti-TNFa-PE-Cy7 BioLgend 502930 MAb11 0.36 ul 0.36 ul 29 Anti-CD107a (LAMP-1) -Biotin BioLgend 328604 H4A3 1ul with 200ul culture medium 2 ul in 200ul culture medium 30 Anti-CD4-CD568 Cytek R7-20041 SK3 0.6 ul 2.5 ul 31 Anti-CD45-PerCP ThermoFisher MHCD4531 HI30 0.5 ul 1.2 ul 32 Anti-CD27-Super Bright 436 ThermoFisher 62-0279-42 O323 5 ul 5 ul 33 Live dead dye-Blue ThermoFisher L23105 0.225 ul 0.225 ul 5. .. Activation induced marker panel Number Reagent Source Catalog number Clone Dilution (PBMC, ul/180ul) Dilution (Adenoid and Tonsil, ul/180ul) 1 Anti-CXCR3-PE/Cy5 BD 551128 1C6/CXCR3 5 ul 5 ul 2 Anti-CXCR5-BV750 BD 747111 RF8B2 1 ul 1 ul 3 Anti-CD45RA-BUV395 BD 740315 5H9 0.6 ul 0.6 ul 4 Anti-ICOS-BUV563 BD 741421 DX29 0.6 ul 1.2 ul 5 Anti-CD56-BUV737 BD 612766 NCAM16.2 3.5 ul 3.5 ul 6 Anti-CD8-BUV805 BD 612889 SK1 1 ul 1.2 ul 7 Anti-CD40L-PE BD 557299 TRAP1 10 ul 15 ul 8 Anti-CD25-PE-CF594 BD 562403 M-A251 2 ul 2 ul 9 Anti-CCR7-BV421 BioLegend 353208 G043H7 1 ul 1 ul 10 Anti-CCR6-BV711 BioLegend 353436 G034E3 1 ul 1 ul 11 Anti-CD4-BV605 BioLegend 317438 OKT4 1 ul 1 ul 12 Anti-CD69-BV650 BioLegend 310934 FN50 2 ul 2.5 ul 13 Anti-PD1-BV785 BioLegend 329930 EH12.2H7 5 ul 1.2 ul 14 Anti-CD57-FITC BioLegend 359604 HNK-1 2 ul 1 ul 15 Anti-CD14-Spark Blue 550 BioLegend 367148 63D3 1 ul 2.5 ul 16 Anti-CD45-PerCP BioLegend 368506 2D1 0.5 ul 1.2 ul 17 Anti-4-1BB-PeCy7 BioLegend 309818 4B4-1 2.5 ul 2.5 ul 18 Anti-CD19-Spark NIR 685 BioLegend 302270 HIB19 1.2 ul 2.5 ul 19 Anti-CD38-APC/Fire 810 BioLegend 303550 HIT2 1 ul 1 ul 20 Anti-HLA-DR-APC/Fire 750 BioLegend 307658 L243 2 ul 2.5 ul 21 Anti-OX40-APC BioLegend 350008 Ber-ACT35 5 ul 5 ul 22 Live dead dye- Blue ThermoFisher L23105 0.9 ul 0.9 ul 23 CD40 Antibody, anti-human Miltenyi 130-094-133 130-094-133 0.5ug/ml 0.5ug/ml 6. .. S1+ /S1- B cell and CD95+ CD4/CD8 T cell sorting panel Number Reagent Source Catalog number Clone Dilution (PBMC, 30mill in 200ul) Dilution (Adenoid & Tonsil, 30mill in 200ul) 1 Anti-CD19-PeCy7 BIoLegend 302216 HIB19 1: 20 1: 20 2 Anti-CD56-BV510 BIoLegend 362533 5.1H11 1: 10 1: 10 3 Anti-CD14-BV510 BIoLegend 367124 63D3 1: 10 1: 10 4 Anti-CD3-Alexa Fluor 488 BIoLegend 317310 OKT3 7: 100 1: 20 5 Anti-CD8-PE BIoLegend 303804 QA18A37 1: 20 1: 20 6 Anti-CD95-PE/Dazzle 594 BIoLegend 305634 DX2 1: 40 1: 40 7 CoV2-S1-Biotin BIoLegend 793806 1: 5 of conjugated antibody 1: 5 of conjugated antibody 8 BIoLegend 405225 9 Anti-CD45-PerCP-Vio 700 Miltenyi 130-110-636 REA747 1: 20 1: 20 10 Anti-CD4-APC-eFLuor 780 ThermoFisher 47-0048-42 OKT4 1: 10 1: 10 11 Live dead dye-Aqua ThermoFisher L34957 7: 200 7: 200 12 SAv-APC ThermoFisher S32362 1: 5 of conjugated antibody 1: 5 of conjugated antibody 7.

    other:

    Article Title: Dynamic stimulation promotes functional tissue-like organization of a 3D human lymphoid microenvironment model in vitro
    Article Snippet: CD40 Antibody, anti-human , Miltenyi , CAT#130-123-798 RRID: AB_2811556.

    Expressing:

    Article Title: Aquaporin-4–Specific T Cell Responses in NMOSD Revealed by mRNA-Engineered Dendritic Cells
    Article Snippet: .. Restimulation was performed for 20-24 h in the presence of CD40-antibody (1μg/ml, Miltenyi Biotec, 130-094-133) to capture transient CD154 expression for surface staining . ..

    Staining:

    Article Title: Aquaporin-4–Specific T Cell Responses in NMOSD Revealed by mRNA-Engineered Dendritic Cells
    Article Snippet: .. Restimulation was performed for 20-24 h in the presence of CD40-antibody (1μg/ml, Miltenyi Biotec, 130-094-133) to capture transient CD154 expression for surface staining . ..

    Generated:

    Article Title: Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity.
    Article Snippet: .. Following a 24 h resting period in cRPMI without supplementation, T cells were again cocultured with a lymphoblastoid cell line (LCL) (Moosmann et al., 2002), generated from the same female donor, in a ratio of 1:10 and supplemented with CD40 antibody (Miltenyi Biotec) to prevent CD40L (CD154) internalization. ..

    Incubation:

    Article Title: Discordant Outcomes of SARS-CoV-2 Exposure in Household Contacts
    Article Snippet: .. Briefly, 1 × 106 cells were incubated with CD40 antibody (Miltenyi Biotech) at 37 ◦C for 15 min and then stimulated with 1 μg/mL of each of the different stimuli at 37 ◦C overnight. .. The following day, Golgi-Plug (brefeldin A; BD Biosciences, CA) and Golgi-stop (monensin; BD Biosciences) were added to the cells and incubated for an additional 4 h. After washing with PBS (Gibco), cells were incubated with antibody cocktails for surface-staining: Live/Dead eFlour 506 Nomenclature AIM Activation Induced Marker CCC Common Cold Coronaviruses CD4RE Experimentally defined CD4 non-spike Epitopes CI Confidence Interval CoV Coronavirus COVID-19 Coronavirus Disease 2019 CP Confirmed Positive DMSO Dimethyl Sulfoxide DP Discordant Pairs ELISA Enzyme-Linked Immunosorbent Assay FBS Fetal Bovine Serum GFP Green Fluorescent Protein HC Healthy Controls HCoV Human Coronavirus HLA Human Leukocyte Antigen ICS Intracellular Staining IFN Interferon Ig Immunoglobulin IL Interleukin IU International Units LOD Limit of Detection LOS Limit of Sensitivity MPs Megapools mRNA Messenger RNA PBMCs Peripheral Blood Mononuclear Cells PBS Phosphate Buffered Saline PCR Polymerase Chain Reaction PFA Paraformaldehyde PHA Phytohemagglutinin-L PMA Phorbol 12-myristate 13-acetate PSV Pseudovirus RBD Receptor Binding Domain RdRp RNA-dependent RNA polymerase SARS-CoV-2 Severe Acute Respiratory Syndrome Coronavirus 2 SD Standard Deviation TMB 3,3′,5,5′-tetramethylbenzidine TNF Tumor Necrosis Factor VSV Vesicular Stomatitis Virus (Thermo Fisher), CD3 BUV805 (UCHT1, BD Biosciences), CD8 BV650 (RPA-T8, Biolegend), CD19 V500 (HIB19, BD Biosciences), CD14 V500 (M5E2, BD Biosciences), CD4 BV605 (RPA-T4, BD Biosciences), OX40 PE-Cy7 (Ber-ACT35, Biolegend), CD137 APC (4B4–1, Biolegend), and CD69 PE (FN50, BD Biosciences) for 30 min at 4 ◦C.



    Similar Products

    anti cd40  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti cd40
    Anti Cd40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pm42398735-96-66-69
    Average 96 stars, based on 1 article reviews
    anti cd40 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti mouse cd40 antibody  (MedChemExpress)
    94
    MedChemExpress anti mouse cd40 antibody
    The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
    Anti Mouse Cd40 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/Anti-Mouse+CD40+Antibody/pmc13429555-23-8-20
    Average 94 stars, based on 1 article reviews
    anti mouse cd40 antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    cd40 antibody, anti-human  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd40 antibody, anti-human
    The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
    Cd40 Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/custom%40130-123-952%4042262895
    Average 96 stars, based on 1 article reviews
    cd40 antibody, anti-human - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    cd40 antibody  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd40 antibody
    The <t>CD40</t> agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant
    Cd40 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/bio_rxiv__64898__2026__06__05__730365-105-10-12
    Average 96 stars, based on 1 article reviews
    cd40 antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    agonistic antibodies against cd40  (Miltenyi Biotec)
    96
    Miltenyi Biotec agonistic antibodies against cd40
    CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
    Agonistic Antibodies Against Cd40, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pmc13275367-71-11-20
    Average 96 stars, based on 1 article reviews
    agonistic antibodies against cd40 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti cd40 blocking antibody  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti cd40 blocking antibody
    CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
    Anti Cd40 Blocking Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/10__1016_slash_j__isci__2026__116175-342-29-32
    Average 96 stars, based on 1 article reviews
    anti cd40 blocking antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti human miltenyi biotec cat  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti human miltenyi biotec cat
    CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
    Anti Human Miltenyi Biotec Cat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pm42167232-239-38-39
    Average 96 stars, based on 1 article reviews
    anti human miltenyi biotec cat - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    cd40 blocking antibody  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd40 blocking antibody
    CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human <t>CD40</t> and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.
    Cd40 Blocking Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+antibody/CD40+Antibody%2C+anti-human/pm41998201-118-6-9
    Average 96 stars, based on 1 article reviews
    cd40 blocking antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    The CD40 agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant

    Journal: Annals of Hematology

    Article Title: Identification and validation of a prognostic risk-scoring model in drug-resistant ALL and evaluation of immune micro-environment

    doi: 10.1007/s00277-026-07080-3

    Figure Lengend Snippet: The CD40 agonist combination therapy effectively inhibits T-ALL by remodeling the tumor microenvironment and establishing immune memory. ( A - B ) The CCK-8 assay was used to evaluate the viability of T-ALL cells under different treatment conditions. ( C ) qRT-PCR detection of transcriptional levels of pro-inflammatory factor genes. ( D ) ELISA assay for the level of granzyme B in serum. ( E ) An intuitive diagram of the size of the mice spleen. ( F ) Flow cytometry for detecting the cell ratios of CCR7 + CD62L +. ( G ) Survival curve graph recorded from the date of re-injection of Jurkat/DNR cells for the group of mice with tumor recurrence. Con, control. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ns, no significant

    Article Snippet: Phorbol 12-myristate 13-acetate (PMA, HY-18739), Daunorubicin hydrochloride (HY-13062), anti-mouse CD40 antibody (FGK45, HY-P990680) and anti-Human PD-L1 (HY-108730) were obtained from MedchemExpress.

    Techniques: CCK-8 Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Injection, Control

    CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human CD40 and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.

    Journal: Frontiers in Immunology

    Article Title: Staphylococcus aureus serine protease-like protein B elicits a type 1/type 2 immune response in atopic dermatitis patients

    doi: 10.3389/fimmu.2026.1798583

    Figure Lengend Snippet: CD4 + T cells in the circulation react to SplB. PBMCs were isolated from healthy donors and AD patients. PBMCs (1 × 10 6 ) were stimulated with SplB in the presence of anti-human CD40 and anti-human CD28 for 24 h. (A) Expression of CD154 on CD4 + T cells with or without stimulation with SplB gated on live CD4 + T cells. (B) SplB-reactive, CD154 + T cells per 1 × 10 5 CD4 + T cells in healthy adults (n = 13, blue) and AD patients (n = 27, yellow); CD154 + T cell frequencies in unstimulated control cells were subtracted. P-values are based on Mann–Whitney U test. Median with interquartile range are depicted. ( C–F ) CD154 + CD4 + T cells were characterized by surface expression of CCR4, CCR10, CCR6, and CXCR3 to define (C) CD154 + Th1 (CXCR3 + CCR4 − CCR10 − CCR6 − ), (D) CD154 + Th2 (CXCR3 − CCR4 + CCR10 − CCR6 − ), (E) CD154 + Th17 (CXCR3 − CCR4 + CCR10 − CCR6 + ), and (F) CD154 + Th22 (CXCR3 − CCR4 + CCR10 + CCR6 + ) cells. Frequencies within 1 × 10 5 CD4 T cells are shown. Friedman test with Dunn’s multiple comparison test. (G) Th2/Th1 and (H) Th2/Th17 ratio of SplB reactive CD4 + T cells in healthy individuals and AD patients with mild (SCORAD <25), moderate (SCORAD 25–50) and severe disease (SCORAD >50). Each data point reflects a single patient. Kruskal–Wallis test with Dunn’s multiple comparison test. TT, tetanus toxoid, SEB, S. aureus enterotoxin (B) Median with interquartile range are depicted.

    Article Snippet: To prevent CD154 internalization and provide co-stimulation and a survival signal, agonistic antibodies against CD40 (1 μg/mL, 130-094-133, clone HB14, Miltenyi, Bergisch Gladbach, Germany) and CD28 (0.5 μg/mL, 555726, clone CD28.2, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) were added for 24 h. To investigate SplB-specific T cells, PBMCs were stained with antibodies (unless otherwise stated: from BioLegend, San Diego, CA, USA): PerCP-conjugated anti-human CD4 (317432, clone OKT4) or PE-Cy5-conjugated anti-human CD4 (555348, clone RPA-T4, Becton Dickinson GmbH, Franklin Lakes, NJ, USA) BV510-conjugated anti-human CD14 (301842, clone M5E2), BV510-conjugated anti-human CD19 (302242; clone HIB19), FITC-conjugated anti-human CD154 (310804; clone 24-31), APC-conjugated anti-human CXCR3 (FAB160A, clone 49801, R&D Systems Inc, Minneapolis, MN, USA), BV421-conjugated anti-human CCR4 (359414, clone L291H4), PE-Cy7-conjugated anti-human CCR6 (353418, clone G034E3), and PE-conjugated anti-human CCR10 (130-120-407, clone: REA326, Miltenyi, Bergisch Gladbach, Germany) ( ).

    Techniques: Isolation, Expressing, Control, MANN-WHITNEY, Comparison